SAXG 01312 - Replicative DNA helicase loader DnaI

SAXG 01312 - Replicative DNA helicase loader DnaI

Rv0004 is a new essential member of the mycobacterial DNA

Using a combination of genetic and biochemical approaches, we found that Rv0004 has a role in DNA replication, interacts with DNA and the replicative helicase DnaB, and affects DnaB-DnaA complex formation. We also identify a conserved domain in Rv0004 that is predicted to structurally resemble the N-terminal protein-protein interaction domain

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Replicative Helicase Loaders: Ring Dispatch Breakers and

[12], contacts DNA and acts like a helicase loader by holding gp4A next to DNA and taking advantage of the transient parting of one of the subunit interfaces to insert DNA into the central channel [5]. The replicative helicase in eukaryotic cells is thought to be the heterohexameric Mcm2-7 complex or a sub-complex thereof [13].

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Site-directed mutagenesis reveals roles for conserved

Site-directed mutagenesis studies on conserved amino acid residues within motifs H1, H1a, H2 and H3 of the hexameric replicative helicase DnaB from Bacillus stearothermophilus revealed specific functions associated with these residues. In particular,

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The Bacterial DnaC Helicase Loader Is a DnaB Ring Breaker

Frontiers | New Insights Into DNA Helicases as Druggable

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Loading and activation of DNA replicative helicases: the

The most characterized replicative DNA helicase is the Escherichia coli DnaB homohexamer. The N-terminal domain of the DnaB protein acts in primase binding, and the C-terminal domain with Walker A and B motifs, and arginine finger inside engages with ATP and associates with the DnaC helicase loader (Yu et al. 1996; Lo et al.

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Crystal structure of the complex of the interaction

Frontiers | New Insights Into DNA Helicases as Druggable

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Molecular interplay between the replicative helicase DnaC

Nov 13, 2009 · Molecular interplay between the replicative helicase DnaC and its loader protein DnaI from Geobacillus kaustophilus. Tsai KL(1), Lo YH, Sun YJ, Hsiao CD. Author information: (1)Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 115, ROC.

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functional interaction between the putative primosomal

Feb 15, 2002 · If DnaI plays the role of the helicase loader in Bacillus then it should be able to interact with the main replicative hexameric helicase. Although an interaction between DnaI and the replicative helicase was detected previously in B.subtilis by yeast two-hybrid technology ( 18 ), no formation of a stable complex between the two proteins has

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Dnai | GE Healthcare | Bioz

( B ) The DnaC helicase activity was detectable only in the presence of the putative DnaI helicase loader. Similar helicase reactions were carried out as above in the presence of DnaI (125, 250, 500, 1000, 2000–4000 nM monomeric). Data were plotted as a percentage of the radiolabelled oligonucleotide displacement versus DnaI concentration.

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RO1_36690 - Replicative DNA helicase loader DnaI

UniProtKB. x; UniProtKB. Protein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects.

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ATPase activity measurement of DNA replicative helicase

The DnaB helicase from Bacillus stearothermophilus (DnaBBst) was a model protein for studying the bacterial DNA replication. In this work, a non-radioactive method for measuring ATPase activity of DnaBBst helicase was described. The working parameters and conditions were optimized. Furthermore, this …

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Viral hijacking of a replicative helicase loader and its

May 31, 2016 · The viral protein binds to the loader's AAA+ ATPase domain, allowing binding of the host replicative helicase but impeding loader self-assembly and ATPase activity. Close inspection of the complex highlights an unexpected locus for the binding of an interdomain linker element in DnaI/DnaC-family proteins.

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Viral hijacking of a replicative helicase loader and its

DNA2 - DNA replication ATP-dependent helicase/nuclease

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A Two-Protein Strategy for the Functional Loading of a

Apr 01, 2003 · The delivery of a ring-shaped hexameric helicase onto DNA is a fundamental step of DNA replication, conserved in all cellular organisms. We report the biochemical characterization of the bacterial hexameric replicative helicase DnaC of Bacillus subtilis with that of the two replication initiation proteins DnaI and DnaB. We show that DnaI and DnaB interact …

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Viral hijacking of a replicative helicase loader and its

May 31, 2016 · Viral hijacking of a replicative helicase loader and its implications for helicase loading control and phage replication. 1 Coronavirus: Find the latest articles and preprints

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DNA replication licensing factor MCM4 - Homo sapiens (Human)

Loading the bacterial replicative helicase DnaB onto DNA requires a specific loader protein, DnaC/DnaI, which creates the loading-competent state by opening the DnaB hexameric ring. To understand the molecular mechanism by which DnaC/DnaI opens the DnaB ring, we solved 3.1-Å co-crystal structure of …

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DciA is an ancestral replicative helicase operator

Delivery of the replicative helicase onto DNA is an essential step in the initiation of replication. In bacteria, DnaC (in Escherichia coli) and DnaI (in Bacillus subtilis) are representative of

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A Two-Protein Strategy for the Functional Loading of a

The delivery of a ring-shaped hexameric helicase onto DNA is a fundamental step of DNA replication, conserved in all cellular organisms. We report the biochemical characterization of the bacterial hexameric replicative helicase DnaC of Bacillus subtilis with that of the two replication initiation proteins DnaI and DnaB. We show that DnaI and DnaB interact physically and …

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DNA2 - DNA replication ATP-dependent helicase/nuclease

Replicative helicases are essential proteins that unwind DNA in front of replication forks. Their loading depends on accessory proteins and in bacteria, DnaC and DnaI are well characterized loaders. However, most bacteria do not express either of these two proteins. Instead, they are proposed to rel …

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